The Extraction and Purification of DNA.
Deoxyribonucleic Acid, popularly known as DNA is an element found in any living thing. This molecule is known to contain a genetic code for every organism hence it is a blueprint that continuously acts in plans of any substance that is produced by these organisms at the course of their living. As much as the molecule has a popular usage, very few individuals have actually seen this molecule; actually the molecule happens to be too small for one to see. However, one can carry out easy experiments right at home or in school using some household materials to extract DNA molecules even from plants like peas.
Some of the crucial materials that you may require for the process include alcohol, meat tenderizer, dishwashing detergent mostly in liquid form, a blender, a beaker or rather clear glass container, a stirring rod or a spoon. In the first step, you need to blend at least a half cup of split peas in a cupful of water. Having obtained a soupy mixture from the blend, use a homogenous solution to separate the mixture. You may as well consider sparing some of the mixtures just in case. Then, pour some of the solutions you have, an adequate amount of it into a container up to a level of at least two inches.
The third procedure involves adding at least one tablespoonful of the liquid dishwashing detergent on top of the solution. Again stir the mixture slowly for about five minutes. In the next procedure; add a pinch of the meat tenderizer on the mixture then stir for another five minutes. Drizzle some alcohol above the solution after stirring. Consider adding the alcohol until you obtain a layer of three inches on the split peas solution. Peas are particularly considered during the extraction because of their high source of this molecule. Again the detergent is used to break down the membrane cell for it to release its contents and the meat tenderizer to dissolve the membrane nuclear for it to release the DNA inside. The alcohol is used in the process to bring out the DNA strands.
The purification procedure can utilize various processing kits. This is because, different samples work with different kind of tissues, forensic samples, plant parts and also cellular structure. Continuously, there are new inventions being made on machines and cutting edge products through different forms of amplification and facilitations for use in new projects and experiments. One such way involves use of magnetized beads which happens to be very effective. Another way involves the use of plasmid purification kits which is able to give low endotoxin levels for use in the subject transfection.